Quantitative spectrophotometric estimation of specific monosaccharides by DNSA method

Abstract

The determination of saccharide is widespread and has led to the development of numerous
analytical techniques for measuring its concentrations. Analytical tool with various applications
in different fields are tedious and have time-consuming protocols ranging from food analysis
to biology. The overall purpose of this research is to develop easy methods for analyzing
reducing sugars or saccarides. The most abundant monosaccharide in nature Dglucose
sometimes referred to as dextrose in our research its shows a peak difference of 1nm as one
water molecule is always loosely attached to dextrose ring structure. In this article it is seen
that DNSA methods has advantage of its simplicity, low cost, sensitivity and adoptable
during handling the analysis of a large number of samples at a time.