Prevention of Solution Leakage during Polyacrylamide Gel Preparation

Guijin Jiang; Hanxiao Zhao; Hongxing Liu; Ying Lin; Yanlong Shen; Xianyu Yang

doi:10.53555/bs.v4i9.2427

Guijin Jiang College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China || School of Forestry and Biotechnology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China
Hanxiao Zhao College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China
Hongxing Liu College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China
Ying Lin College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China || Zhejiang Biosan Biochemical Technologies Co. Ltd., 77 Xueyuan Road, Hangzhou, Zhejiang 310012, China
Yanlong Shen College of Animal Science and Technology, Zhejiang A&F University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China || Shanghai Ruidi Biological Technology Co. Ltd., 698 Changjiang South Road, Shanghai 200431, China
Xianyu Yang

DOI: https://doi.org/10.53555/bs.v4i9.2427

Keywords: Agarose gel sealing, Polyacrylamide gel, Prevention of solution leakage

Abstract

Polyacrylamide gel electrophoresis (PAGE) is an essential technique in molecular biology. However, solution leakage often leads to the failure of PAGE gel preparation. Here a simple method is reported to prevent the leakage for mini PAGE gel preparation by filling 400 μl of 1% hot agarose solution from the bottom of the assembled glass plates. This method makes almost no solution leakage during acrylamide polymerization ensuring the successful PAGE gel preparation and no bubbles trapped beneath the bottom of the gel having to be removed before the initiation of the electrophoresis.

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References

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Prevention of Solution Leakage during Polyacrylamide Gel Preparation

Abstract

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